|
Status |
Public on Dec 01, 2012 |
Title |
heart_Scn5a-1798insD/+ mutant [4700266023_G] |
Sample type |
RNA |
|
|
Source name |
left ventricle_free-wall
|
Organism |
Mus musculus |
Characteristics |
tissue: cardiac background strain: F2-(129P2/OlaHsdxFVBN/J) genotype: Scn5a1798insD/+
|
Treatment protocol |
None
|
Growth protocol |
All mice were supplied with the same SDS diet (SDS CRM(E) PL; Special Diets Services, UK) and water ad libitum and maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the QIAGEN RNeasy mini kit. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
|
Label |
biotin
|
Label protocol |
Synthesis, amplification and purification of anti-sense RNA was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion) following the Illumina Sentrix Array Matrix expression protocol.
|
|
|
Hybridization protocol |
A total of 750 ng biotinylated cRNA was hybridized in accordance with standard Illumina protocols.
|
Scan protocol |
Standard Illumina scan protocol
|
Description |
F2 hybrid
|
Data processing |
Illumina’s default pre-processing steps were performed using beadarray. A variance-stabilization transformation was applied to the summarized data and the resulting data was quantile normalized.
|
|
|
Submission date |
Feb 11, 2011 |
Last update date |
Dec 01, 2012 |
Contact name |
Brendon Scicluna |
E-mail(s) |
[email protected]
|
Organization name |
University of Malta
|
Street address |
Msida campus
|
City |
Msida |
ZIP/Postal code |
MSD2020 |
Country |
Malta |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE27236 |
Gene expression analysis of cardiac left-ventricle tissue from hybrid mice harboring the Scn5a-1798insD/+ mutation |
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