GEO help: Mouse over screen elements for information.
Status
Public on Mar 05, 2006
Title
diabetic muscle replicate 3
Sample type
RNA
Source name
rear leg muscle
Organism
Rattus norvegicus Characteristics
Diabetic Male Wistar rat after 28 days of diabetes induced with streptozotocin
Extracted molecule
polyA RNA
Extraction protocol
Total RNA was obtained from approximately 100 mg of rear leg skeletal muscle stored in RNAlater® Qiagen (Valencia, CA) at -80oC using Qiagen RNeasy Maxi kits. Homogenates were made using a Brinkman/Kinetmatica Model PT-2100 polytron (Westbury, NY) for tissue dispersal. Total RNA samples were further processed for poly(A)+ mRNA isolation using Qiagen oligotex mRNA kit.
Label
biotin
Label protocol
Approximately 1 ug of mRNA processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Aug 05, 2005
Last update date
Dec 01, 2005
Contact name
Gail R Willsky
E-mail(s)
[email protected] Phone
716-829-2969
Fax
716 829-2725
Organization name
University at Buffalo
Department
Biochemistry
Lab
Willsky
Street address
140 Farber Hall
City
Buffalo
State/province
NY
ZIP/Postal code
14214
Country
USA
Platform ID
GPL85 Series (1)
GSE3068 normal and diabetic rat muscle treated and untreated with vanadyl sulfate
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE A01157cds_s_at
232.3
A
0.234557
A03913cds_s_at
503.8
A
0.218983
A04674cds_s_at
113.4
A
0.715253
A07543cds_s_at
421.5
A
0.47938
A09811cds_s_at
46.9
A
0.980696
A16585cds_s_at
184.7
A
0.438361
A17753cds_s_at
36.5
A
0.901946
A30543cds_s_at
113.8
A
0.67917
A44407cds_at
50.3
A
0.872355
AA108277_at
30
A
0.910631
AA108308_i_at
20.3
A
0.937979
AA108308_s_at
1392.1
A
0.234557
AA684537_at
2315.7
P
0.02786
AA684929_f_at
738.4
A
0.189687
AA684960_f_at
883.7
A
0.397994
AA684963_at
3785.6
P
0.008689
AA685112_at
3405.9
P
0.000388
AA685152_r_at
52.6
A
0.904785
AA685376_f_at
118.8
A
0.781017
AA685876_at
1638
A
0.11716
8799 259 Kbytes .Supplementary data files not provided
