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Sample GSM67419

Query DataSets for GSM67419

Status

Public on Mar 05, 2006

Title

diabetic muscle from vanadyl sulfate treated rats replicate 2

Sample type

RNA

Source name

rear leg muscle

Organism

Rattus norvegicus

Characteristics

Diabetic Male Wistar rat after 7 days of diabetes induced with streptozotocin and subsequent treatment with oral vanadyl sulfate for 20 days.

Extracted molecule

polyA RNA

Extraction protocol

Total RNA was obtained from approximately 100 mg of rear leg skeletal muscle stored in RNAlater® Qiagen (Valencia, CA) at -80oC using Qiagen RNeasy Maxi kits. Homogenates were made using a Brinkman/Kinetmatica Model PT-2100 polytron (Westbury, NY) for tissue dispersal. Total RNA samples were further processed for poly(A)+ mRNA isolation using Qiagen oligotex mRNA kit.

Label

biotin

Label protocol

Approximately 1 ug of mRNA processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Aug 05, 2005

Last update date

Dec 01, 2005

Contact name

Gail R Willsky

E-mail(s)

[email protected]

Phone

716-829-2969

Fax

716 829-2725

Organization name

University at Buffalo

Department

Biochemistry

Lab

Willsky

Street address

140 Farber Hall

City

Buffalo

State/province

ZIP/Postal code

14214

Country

USA

Platform ID

GPL85

Series (1)

GSE3068

normal and diabetic rat muscle treated and untreated with vanadyl sulfate

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A01157cds_s_at	169.6	A	0.47938
A03913cds_s_at	1794.1	P	0.000805
A04674cds_s_at	109.4	A	0.765443
A07543cds_s_at	307.7	A	0.52062
A09811cds_s_at	76.1	A	0.933135
A16585cds_s_at	361.6	A	0.189687
A17753cds_s_at	45.6	A	0.849473
A30543cds_s_at	60.3	A	0.697453
A44407cds_at	66.7	A	0.849473
AA108277_at	240.2	A	0.098054
AA108308_i_at	10.7	A	0.972234
AA108308_s_at	1889.8	A	0.234557
AA684537_at	6432.8	P	0.007543
AA684929_f_at	1596.1	M	0.043968
AA684960_f_at	2026.3	A	0.07383
AA684963_at	2056.2	P	0.013092
AA685112_at	6642.2	P	0.000219
AA685152_r_at	301.6	A	0.665527
AA685376_f_at	971.7	A	0.302547
AA685876_at	2967.5	M	0.05447

Total number of rows: 8799

Table truncated, full table size 259 Kbytes.

Supplementary data files not provided