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Sample GSM67420

Query DataSets for GSM67420

Status

Public on Mar 05, 2006

Title

diabetic muscle from vanadyl sulfate treated rats replicate 3

Sample type

RNA

Source name

rear leg muscle

Organism

Rattus norvegicus

Characteristics

Diabetic Male Wistar rat after 7 days of diabetes induced with streptozotocin and subsequent treatment with oral vanadyl sulfate for 20 days.

Extracted molecule

polyA RNA

Extraction protocol

Total RNA was obtained from approximately 100 mg of rear leg skeletal muscle stored in RNAlater® Qiagen (Valencia, CA) at -80oC using Qiagen RNeasy Maxi kits. Homogenates were made using a Brinkman/Kinetmatica Model PT-2100 polytron (Westbury, NY) for tissue dispersal. Total RNA samples were further processed for poly(A)+ mRNA isolation using Qiagen oligotex mRNA kit.

Label

biotin

Label protocol

Approximately 1 ug of mRNA processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Aug 05, 2005

Last update date

Dec 01, 2005

Contact name

Gail R Willsky

E-mail(s)

[email protected]

Phone

716-829-2969

Fax

716 829-2725

Organization name

University at Buffalo

Department

Biochemistry

Lab

Willsky

Street address

140 Farber Hall

City

Buffalo

State/province

ZIP/Postal code

14214

Country

USA

Platform ID

GPL85

Series (1)

GSE3068

normal and diabetic rat muscle treated and untreated with vanadyl sulfate

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A01157cds_s_at	68.7	A	0.32083
A03913cds_s_at	454.6	A	0.250724
A04674cds_s_at	76.8	A	0.892699
A07543cds_s_at	549.4	A	0.418056
A09811cds_s_at	94	A	0.901946
A16585cds_s_at	123.8	A	0.418069
A17753cds_s_at	49.5	A	0.872355
A30543cds_s_at	158.2	A	0.660442
A44407cds_at	60.8	A	0.872355
AA108277_at	19.1	A	0.939581
AA108308_i_at	17.6	A	0.945289
AA108308_s_at	1573.4	A	0.32083
AA684537_at	3034.9	P	0.02786
AA684929_f_at	1223.7	A	0.189687
AA684960_f_at	1261.3	A	0.32083
AA684963_at	2115.7	P	0.013092
AA685112_at	3699.5	P	0.000468
AA685152_r_at	83.8	A	0.753906
AA685376_f_at	429.4	A	0.602006
AA685876_at	1245.3	A	0.378184

Total number of rows: 8799

Table truncated, full table size 259 Kbytes.

Supplementary data files not provided