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Status
Public on Mar 05, 2006
Title
normal muscle from vanadyl sulfate treated rats replicate 2
Sample type
RNA
Source name
rear leg muscle
Organism
Rattus norvegicus Characteristics
Normal Male Wistar rat treated with oral vanadyl sulfate for 28 days.
Extracted molecule
polyA RNA
Extraction protocol
Total RNA was obtained from approximately 100 mg of rear leg skeletal muscle stored in RNAlater® Qiagen (Valencia, CA) at -80oC using Qiagen RNeasy Maxi kits. Homogenates were made using a Brinkman/Kinetmatica Model PT-2100 polytron (Westbury, NY) for tissue dispersal. Total RNA samples were further processed for poly(A)+ mRNA isolation using Qiagen oligotex mRNA kit.
Label
biotin
Label protocol
Approximately 1 ug of mRNA processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Aug 05, 2005
Last update date
Dec 01, 2005
Contact name
Gail R Willsky
E-mail(s)
[email protected] Phone
716-829-2969
Fax
716 829-2725
Organization name
University at Buffalo
Department
Biochemistry
Lab
Willsky
Street address
140 Farber Hall
City
Buffalo
State/province
NY
ZIP/Postal code
14214
Country
USA
Platform ID
GPL85 Series (1)
GSE3068 normal and diabetic rat muscle treated and untreated with vanadyl sulfate
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE A01157cds_s_at
113
A
0.52062
A03913cds_s_at
1525.1
P
0.001892
A04674cds_s_at
351.4
A
0.660442
A07543cds_s_at
629.1
A
0.458816
A09811cds_s_at
116.7
A
0.933135
A16585cds_s_at
310.7
A
0.284747
A17753cds_s_at
56.2
A
0.910595
A30543cds_s_at
144.1
A
0.781017
A44407cds_at
71.5
A
0.956032
AA108277_at
318.9
P
0.02786
AA108308_i_at
25.3
A
0.937979
AA108308_s_at
4071.5
A
0.189687
AA684537_at
4471.1
A
0.066865
AA684929_f_at
1873.9
A
0.098054
AA684960_f_at
2090
A
0.162935
AA684963_at
1788.6
A
0.07383
AA685112_at
6697.3
P
0.000219
AA685152_r_at
115.1
A
0.904785
AA685376_f_at
701.4
A
0.438361
AA685876_at
2599.5
A
0.150527
8799 259 Kbytes .Supplementary data files not provided
