|
| Status |
Public on Jan 11, 2013 |
| Title |
4 weeks transfer-G44 |
| Sample type |
RNA |
| |
|
| Source name |
colon
|
| Organism |
Mus musculus |
| Characteristics |
tissue: colon
treatment: T cell transfer
time: 4 weeks
genotype: RAG-1-/-
genetic background: C57BL/6
|
| Treatment protocol |
Half a million CD4+CD45RBhigh T cells from healthy wild type C57BL/6 male mice were transferred to male (Rag-/-) mice on C57BL/6 background. The mice were sacrificed and the colon tissue were collected at week 0, 2, 4 and 6.
|
| Growth protocol |
The mouse were housed in specific pathogen-free conditions according to Institutional Animal Care and Use Committee (IACUC) regulations and the National Research Council's Guide for the Care and Use of Laboratory Animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using Rneasy isolation kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 500 as normalization method.
|
| |
|
| Submission date |
Feb 14, 2011 |
| Last update date |
Jan 11, 2013 |
| Contact name |
Kevil G Christopher |
| E-mail(s) |
[email protected]
|
| Phone |
3186754694
|
| Organization name |
Louisiana State University Health Science Center, Shreveport
|
| Department |
Pathology
|
| Street address |
1501 Kings Highway
|
| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71105 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27302 |
Expression data from mouse colon tissue response to T cell transfer at week 0, 2, 4 and 6 |
|
