|
| Status |
Public on Apr 27, 2011 |
| Title |
Cyclophosphamide-treated spleen 1 day - Biological Replicate 3 - Technical Replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
splenocytes
|
| Organism |
Mus musculus |
| Characteristics |
fresh tissue: splenocytes
strain: DBA/2
|
| Treatment protocol |
Mice were injected i.p. with 83 mg/Kg cyclophosphamide. Splenocytes were harvested 1 day after treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy Midi Kit following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
aRNA were obtained using the Ambion Amino Allyl MessageAmp™ II aRNA Amplification Kit, according to the manufacturer's instructions. 12 µg of aRNA were labeled with Amersham Cy5 (RNA samples) or Cy3 (Reference RNA)
|
| |
|
| Channel 2 |
| Source name |
bone marrow cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: pool of untreated bone marrow cells
strain: DBA/2
sample type: reference
|
| Treatment protocol |
Mice were treated with 83 mg/Kg cyclophosphamide
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy Midi Kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
aRNA were obtained using the Ambion Amino Allyl MessageAmp™ II aRNA Amplification Kit, according to the manufacturer's instructions. 12 µg of aRNA were labeled with Amersham Cy5 (RNA samples) or Cy3 (Reference RNA)
|
| |
|
| |
| Hybridization protocol |
Labeled samples, diluted in Hybridization buffer (5X SSC; 50% Formamide; salmon sperm-DNA 100 ng/ul; 0.1% SDS), were applied to microarrays enclosed in Corning hybridization chambers - 42°C over night.
|
| Scan protocol |
Slides were scanned on GenePix® 4200A instrument (Axon Instruments)
Images were quantified using GenePix Pro 6.0
|
| Description |
Biological replicate 3 of 4. Technical Replicate 1 of 2. Cyclophosphamide-treated splenocytes, day1
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. BRBArrayTools software was used.
|
| |
|
| Submission date |
Feb 18, 2011 |
| Last update date |
Apr 27, 2011 |
| Contact name |
Federica Moschella |
| E-mail(s) |
[email protected]
|
| Phone |
+390649902474
|
| Organization name |
Istituto Superiore di Sanità
|
| Department |
Cell Biology and Neurosciences
|
| Street address |
Viale Regina Elena 299
|
| City |
Roma |
| ZIP/Postal code |
00161 |
| Country |
Italy |
| |
|
| Platform ID |
GPL13209 |
| Series (2) |
| GSE27423 |
Mouse spleen cyclophosphamide treated vs untreated |
| GSE27440 |
Mouse cells cyclophosphamide treated vs untreated |
|
