|
| Status |
Public on Jan 18, 2023 |
| Title |
F2TCDD [TCDD_F2_E2] |
| Sample type |
RNA |
| |
|
| Source name |
Pregnanat (GD14) mice exposed to TCDD
|
| Organism |
Mus |
| Characteristics |
strain: C57BL/6
Sex: female
tissue: Thymus
treatment: exposed to TCDD (10 µg/kg bw)
|
| Treatment protocol |
14GD pregnant mice were exposed to TCDD (10 µg/kg bw)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Mini Kit (Cat No#74104 from Qiagen was used to isolate total RNAs including microRNAs
|
| Label |
Biotin
|
| Label protocol |
FlashTag Biotin HSR RNA labeling Kit from Affymetrix was used.
|
| |
|
| Hybridization protocol |
Afymetrix GeneChip miRNA Arrays kits and their protocol for hybridization, labeling and blocking was used
|
| Scan protocol |
strandard Affymetrix protocol
|
| Description |
Total RNA including microRNAs
Total RNAs/Thymic cells
|
| Data processing |
Command Console was used to scan the samples
Expression Console software was used for data summarization, normalization, and quality control
|
| |
|
| Submission date |
Dec 06, 2022 |
| Last update date |
Jan 18, 2023 |
| Contact name |
Narendra P Singh |
| E-mail(s) |
[email protected]
|
| Phone |
8032163422
|
| Organization name |
University of South Carolina
|
| Department |
Pathology, Microbiology, and Immunology
|
| Street address |
6439 Garners Ferry Road,
|
| City |
Columbia |
| State/province |
South Carolina |
| ZIP/Postal code |
29208 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE220204 |
TCDD-mediated multigenerational effects in mice |
|
