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| Status |
Public on Dec 09, 2022 |
| Title |
wt rep 1 |
| Sample type |
SRA |
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| Source name |
Brain
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Brain
cell type: Cortical neurons
genotype: wild type
age: E16.5
Sex: male
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen RNeasy kit (Qiagen, 74104) according to the manufacturer's instructions
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
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| Description |
2.Wildtype
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| Data processing |
Reads (49bp) were aligned to the mouse genome mm9 using the Rsubread package v1.4.0
Read counts were summarised at the gene level using the featureCounts function in Rsubread V1.4.0 using the in-built RefSeq annotation
Assembly: mm9
Supplementary files format and content: tab delimited text file with entrez gene id and gene length ain the first two columns, followed by 7 columns containing counts for each sample
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| Submission date |
Dec 09, 2022 |
| Last update date |
Dec 09, 2022 |
| Contact name |
Belinda Phipson |
| Organization name |
WEHI
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| Department |
Bioinformatics
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| Lab |
Phipson
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| Street address |
1G Royal Parade
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| City |
Parkville |
| State/province |
VIC |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platform ID |
GPL11002 |
| Series (1) |
| GSE220602 |
Stem cell plasticity, acetylation of H3K14 and de novo gene activation rely on KAT7 |
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| Relations |
| BioSample |
SAMN32127775 |
| SRA |
SRX18550180 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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