|
| Status |
Public on Mar 03, 2011 |
| Title |
liver-young LA-6 |
| Sample type |
RNA |
| |
|
| Source name |
liver-young LA
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Fischer 344
gender: male
tissue: liver
age: 3 months
diet: lipoic acid supplemented
|
| Treatment protocol |
2-week modified pair-feeding with or without 0.2% R-alpha-lipoic acid.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy minikit (Qiagen) according to manufacturer's protocols for liver tissue.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3ug total RNA (Expression Analysis Technical Manual 701021 Rev. 5.)
|
| |
|
| Hybridization protocol |
Following fragmentation 10ug of cRNA were hybridized on Rat Expression 230 2.0 Genechips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned with an Affymetrix GeneChip® Scanner 3000 at 570nm.
|
| Data processing |
Processing of Cel files was performed by GC-RMA.
|
| |
|
| Submission date |
Mar 02, 2011 |
| Last update date |
Mar 03, 2011 |
| Contact name |
Tory Hagen |
| Organization name |
Oregon State University
|
| Department |
Linus Pauling Institute
|
| Lab |
Hagen Lab
|
| Street address |
110 Weniger Hall
|
| City |
Corvallis |
| State/province |
OR |
| ZIP/Postal code |
97330 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE27625 |
Hepatic gene expression changes in aging and R-α-lipoic acid supplementation: Necroinflammatory phenotype, lipid synthesis regulation and circadian rhythms. |
|
