|
| Status |
Public on Mar 03, 2011 |
| Title |
Normal skin from Tie2_control mouse, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Normal skin from Tie2_control mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
tissue type: back skin
|
| Treatment protocol |
For all experiments involving transgenic mice, experimental and control animals were genotyped at weaning using PCR, and development of psoriasiform lesions was spontaneous and not induced by wounding (e.g., tape stripping). For the IMQ-generated phenotype, the treatment protocol was to apply a daily topical dose of 62.5 mg IMQ cream (5% Aldara; 3M Pharmaceuticals) to the shaved back region of 8-10 week old C57BL/6 mice. This treatment was carried out for six consecutive days, and during this time, control animals were treated with a vehicle cream (Vaseline Lanette cream; Fagron). In all experiments, adult mice were euthanized at 10-20 weeks of age at which time ear, tail or back skin samples were flash frozen and stored at -80C prior to the isolation of total RNA.
|
| Growth protocol |
Laboratory animals were maintained under standard conditions in accordance with protocols approved by animal welfare committees at each participating institution
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNeasy mini kits
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
|
| Description |
Normal skin without psoriasiform phenotype
|
| Data processing |
Robust Multichip Average (RMA)
|
| |
|
| Submission date |
Mar 02, 2011 |
| Last update date |
Mar 03, 2011 |
| Contact name |
William R Swindell |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard University
|
| Department |
Genetics
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27628 |
Expression data from affected skin from psoriasis mouse models and normal skin from control mice |
|
