cell type: Human mammary epithelial cells cell line: 184A1
Treatment protocol
184A1 human mammary cells were GF-starved for 16 hours. They were then either pulsed with EGF at time 0 for 1 hour, washed and incubated in starvation medium for 7 hours, followed by a second, one hour long pulse of EGF. Samples are taken at the time point indicated; For the 1st-pulse-only samples, they were then either pulsed with EGF at time 0 for 1 hour, washed and incubated in starvation medium for 7 hours, followed by a mock pulse. Samples are taken at the time point indicated.
One EGF pulse time course experiment, see Figure 3 in the paper for experiment design
Data processing
Normalized expression signals were calculated from Affymetrix CEL files using Affymetrix's Expression Console by RMA (Irizarry et al., 2003). HuGene-1_0-st-v1.r4.pgf HuGene-1_0-st-v1.na28.hg18.transcript.csv
