|
| Status |
Public on May 15, 2011 |
| Title |
LNCaP-RNA-vehicle-siCTRL rep2 [gene expr] |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP-vehicle-siCTRL
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: prostate cancer cell line (LNCaP)
sirna transfection: siCTRL (1027280)
agent: vehicle
|
| Treatment protocol |
Control (1027280) and the specific siRNA against FOXA1 (M-010319) were purchased from Qiagen or Dharmacon. One day prior to transfection, LNCaP cells were seeded in RPMI 1640 medium. Six hours after transfection with Lipofectamine 2000 (Invitrogen), cells were washed twice with PBS and then maintained in hormone-deprived phenol-free RPMI 1640 media. Cells were then cultured for 72 hours following transfection and then treated with DHT or vehicle for 1 hrs. = Quantile normalization with Illumina Inc. BeadStudio version 1.5.1.3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 5 Mio cells on Rneasy columns (Qiagen) with on-column DNase digestion following the manufacturer's instructions. RNA quality was assessed on an Agilent 2100 BioAnalyzer .
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
LNCaP gene expression treated with control siRNA and vehicle for 24h
|
| Data processing |
quantile normalization with Illumina Inc. BeadStudio version 1.5.1.3
|
| |
|
| Submission date |
Mar 04, 2011 |
| Last update date |
May 15, 2011 |
| Contact name |
Christopher Benner |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California, San Diego (UCSD)
|
| Department |
Medicine
|
| Street address |
9500 Gilman Dr. MC 0640
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92093-0640 |
| Country |
USA |
| |
|
| Platform ID |
GPL6102 |
| Series (2) |
| GSE27682 |
Reprogramming Transcriptional Responses through Functionally-Distinct Classes of Enhancers in Prostate Cancer Cells [gene expression] |
| GSE27824 |
Reprogramming Transcriptional Responses through Functionally-Distinct Classes of Enhancers in Prostate Cancer Cells |
|
