|
| Status |
Public on May 12, 2011 |
| Title |
LSK WT replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
Lineage negative, cKit+, Sca1+ bone marrow cells from 6 weeks old WT mouse
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
age: 6 weeks
genotype: Ncstn flox/flox
|
| Treatment protocol |
14 days old Ncstn flox/flox and Ncstn flox/flox Mx1-cre+ littermate were injected 3 times with polyIC (20mcg/g of body weight) every other day. Mice were sacrificed and analyzed 4 weeks after injection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
|
| Label |
biotin
|
| Label protocol |
5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
|
| |
|
| Hybridization protocol |
3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
|
| Scan protocol |
Affymetrix 7G GeneArray scanner
|
| Description |
4 weeks after polyIC injection
|
| Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
|
| |
|
| Submission date |
Mar 07, 2011 |
| Last update date |
May 12, 2011 |
| Contact name |
Camille Lobry |
| E-mail(s) |
[email protected]
|
| Organization name |
Inserm
|
| Department |
IRSL Inserm U944 / CNRS UMR7212
|
| Lab |
Lobry lab
|
| Street address |
16 rue de la Grange aux Belles
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE27794 |
Expression data from LSK WT and LSK NcstnKO |
| GSE27833 |
Notch signaling in HSC |
|
