|
| Status |
Public on Sep 06, 2023 |
| Title |
Human meningioma, FFPE [AM9] |
| Sample type |
RNA |
| |
|
| Source name |
Human meningioma, FFPE
|
| Organism |
Homo sapiens |
| Characteristics |
disease: meningioma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen or FFPE meningioma tumor samples using Qiagen AllPrep or Qiagen FFPE RNAeasy kits.
|
| Label |
n/a
|
| Label protocol |
200 ng of total RNA per sample was hybridized to barcoded reporter probes and biotin-conjugated capture probes from a custom codeset targeting genes of interest at 65C for 16 hours according to manufacturer instruction. Hybridization mixtures were washed and target/probe complexes were purified and bound to streptavidin coated cartridges.
|
| |
|
| Hybridization protocol |
200 ng of total RNA per sample was hybridized to barcoded reporter probes and biotin-conjugated capture probes from a custom codeset targeting genes of interest at 65C for 16 hours according to manufacturer instruction. Hybridization mixtures were washed and target/probe complexes were purified and bound to streptavidin coated cartridges.
|
| Scan protocol |
Cartridges were scanned on the nCounter Digital Analyzer with a FOV setting of 550
|
| Description |
Nanostring nCounter
|
| Data processing |
Samples here are unique meningiomas included in the study. Counts were positive control normalized using the spike-in positive control probes in the nSolver solftware, as per recommendations from the manufacturer (Nanostring).
Positive control normalized counts
|
| |
|
| Submission date |
Jan 03, 2023 |
| Last update date |
Sep 06, 2023 |
| Contact name |
William Chen |
| Organization name |
UCSF
|
| Street address |
1450 3rd Street, HD 402
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL32994 |
| Series (1) |
| GSE222054 |
Targeted gene expression panel for meningiomas |
|
