|
Status |
Public on Mar 26, 2011 |
Title |
uninfected control, biological replicate 3 |
Sample type |
RNA |
|
|
Source name |
jejunal tissue collected by upper endoscopy
|
Organism |
Homo sapiens |
Characteristics |
tissue: jejunum disease state: HIV-
|
Treatment protocol |
treatment with highly active antiretroviral therapy was removed
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen Rneasy kit was used to extract total RNA
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000
|
Description |
Gene expression data from jejunal tissue
|
Data processing |
The data were analyzed with dChip
|
|
|
Submission date |
Mar 25, 2011 |
Last update date |
Mar 26, 2011 |
Contact name |
Michael George |
E-mail(s) |
[email protected]
|
Organization name |
University of California, Davis
|
Department |
Medical Microbiology and Immunology
|
Street address |
451 Health Sciences Drive
|
City |
Davis |
State/province |
CA |
ZIP/Postal code |
95616 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE28177 |
Expression data from HAART interruption in HIV patients |
|