|
| Status |
Public on Sep 21, 2013 |
| Title |
paediatric low grade glioma_LGG_26 |
| Sample type |
RNA |
| |
|
| Source name |
tumour sample
|
| Organism |
Homo sapiens |
| Characteristics |
lesion site: supratentorial
|
| Treatment protocol |
Tumour samples were included in HistoGel prior to dissection in 10 μm slices
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from serial frozen sections of tumour tissue by using the TRIzol reagent combined with silica column purification system
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed according Affymetrix GeneChip Expression Analysis Technical Manual
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
| Description |
Gene expression data from pediatrics low grade gliomas
|
| Data processing |
Gene expressions were extracted from the .CEL files and normalized using the Robust Multichip Average method by running an R script based on the aroma package.
|
| |
|
| Submission date |
Mar 29, 2011 |
| Last update date |
Sep 21, 2013 |
| Contact name |
annalisa barla |
| E-mail(s) |
[email protected]
|
| Phone |
+390103536602
|
| Organization name |
Università degli Studi di Genova
|
| Department |
DIBRIS
|
| Lab |
Slipguru
|
| Street address |
via Dodecaneso 35
|
| City |
Genova |
| State/province |
GE |
| ZIP/Postal code |
I-16146 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
|
