|
| Status |
Public on Oct 15, 2011 |
| Title |
Renal medulla miRNA expression of normotensive, m38D miR |
| Sample type |
RNA |
| |
|
| Source name |
Renal medulla of normotensive male
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: renal medulla
blood pressure: normotensive
|
| Treatment protocol |
Tissue was transferred into containers with RNAlater (Ambion) and preserved at 70°C before mRNA extraction.
|
| Growth protocol |
Approximately 1 cm3 of tissue from the healthy (unaffected by cancer) pole of the kidney was obtained immediately after surgery.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNeasy kit (Qiagen) was used for extraction of total RNA according to the manufacturer's instructions. Medulla and cortex were separated and individual RNA was obtained for each of them.
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA was prepared for hybridization according to the standard Agilent protocol (microRNA Labeling & Hybridization Kit Manual, Agilent, Version 1.7, October 2009).
|
| |
|
| Hybridization protocol |
After fragmentation, samples were hybridized to Agilent Human miRNA Microarrays (V3, release 12.0) for 20 hr at 55°C. Chips were washed and stained in the Agilent Microarray Hybridization Chamber SureHyb. The Agilent’s miRNA Complete Labeling and Hyb Kit was used according to Agilent's manual.
|
| Scan protocol |
The chips were scanned on an Agilent G2565BA scanner.
|
| Description |
miRNA expression from male normotensive patient.
|
| Data processing |
The samples were between-array normalized so that expression intensities were similar across all arrays using the quantile method, in the R application (version 2.11.1). Differentially expressed miRNAs were identified using the moderated two-sample t-test in the Limma package.
|
| |
|
| Submission date |
Apr 03, 2011 |
| Last update date |
Oct 15, 2011 |
| Contact name |
Prof.Brian J Morris |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Sydney
|
| Department |
Physiology
|
| Lab |
Basic & Clinical Genomics Lab
|
| Street address |
Anderson Stuart Building, room N452, University of Sydney
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL10850 |
| Series (2) |
| GSE28260 |
Renal cortex and medulla microRNA and mRNA expression differences between hypertensive and normotensive patients |
| GSE28344 |
Renal medulla microRNA expression differences between hypertensive and normotensive patients |
|
