|
| Status |
Public on Apr 04, 2011 |
| Title |
SR1_100nM_24hr |
| Sample type |
RNA |
| |
|
| Source name |
SR1_100nM_24hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hematopoietic stem cell (HSC)
agent: SR1
dose: 100nM
time: 24hr
|
| Treatment protocol |
mPB CD34+ cells treated with compounds at the indicated time points
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed by using RNeasy kits(Qiagen). The integrity and concentration of RNA was determined by microfluidic analysis on an Experion instrument (BioRad)
|
| Label |
biotin
|
| Label protocol |
GeneChip 3' IVT Express kits (Affymetrix) were used to amplify cRNA from 500 ng total RNA.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
SR1_100nM_24hr
|
| Data processing |
gcRMA
|
| |
|
| Submission date |
Apr 04, 2011 |
| Last update date |
Apr 04, 2011 |
| Contact name |
John R Walker |
| E-mail(s) |
[email protected]
|
| Phone |
858-812-1636
|
| Organization name |
Genomics Institute of the Novartis Research Foundation
|
| Lab |
Genetics Core
|
| Street address |
10675 John Jay Hopkins
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28359 |
Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells |
|
