|
| Status |
Public on Apr 14, 2011 |
| Title |
Chromatin accessibility assay of CD8 Primary Cells; DS17332 |
| Sample type |
SRA |
| |
|
| Source name |
flow sorted CD8+ Cells, year 34; DS17332
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: CD8.RO_01727
sample common name: CD8 Primary Cells
molecule: genomic DNA
disease: None
biomaterial_provider: FHCRC HEIMFELD
biomaterial_type: Primary Cell
cell_type: CD8 Primary Cells
markers: CD8+
donor_id: RO 01689
donor_age: year 34
donor_health_status: NA
donor_sex: Female
donor_ethnicity: Caucasian
passage_if_expanded: NA
experiment_type: Chromatin Accessibility
extraction_protocol: Qiagen minElut
dnase_protocol: Stamlab DNase Protocol, Sabo, P. J. et al. Nat Methods 3, 511-518 (2006)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
DNase-Hypersensitivity |
| Library source |
genomic |
| Library selection |
DNAse |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
sample_term_id: CL_0000625
assay_term_id: OBI_0001853
nucleic_acid_term_id: SO_0000352
Design description: Chromatin accessibility determination via DNase-seq
Library name: DS17332
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FEXPERIMENT%2FEDACC.7032
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.7031
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM701499_UW.CD8_Primary_Cells.ChromatinAccessibility.RO_01689.DS17332.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DS17332.hg19.level.1.release.4
ANALYSIS TITLE: Mapping of CD8 Primary Cells Chromatin Accessibility Data
ANALYSIS DESCRIPTION: Illumina reads produced by DNAse-Seq on CD8 Primary Cells, Donor RO 01689, Library DS17332 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7725
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: Chromatin Accessibility
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: None
ALIGNMENT_POSTPROCESSING: None
RELEASE_NUMBER: Human Epigenome Atlas 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 25,588,179
NUMBER_OF_CHROMATIN_ACCESSIBILITY_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 54
FINDPEAKS_SCORE: 0.2729
FINDPEAKS_PERCENTILE: 13
HOTSPOT_SCORE: 0.2739
HOTSPOT_PERCENTILE: 11
IROC_SCORE: 0.9955
IROC_PERCENTILE: 63
POISSON_SCORE: 0.3195
POISSON_PERCENTILE: 9
MAXIMUM_REPLICATE_CORRELATION: 0.93
**********************************************************************
ANALYSIS FILE NAME: GSM701499_UW.CD8_Primary_Cells.ChromatinAccessibility.RO_01689.DS17332.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DS17332.hg19.level.2.release.4
ANALYSIS TITLE: Raw Signal Density Graphs of CD8 Primary Cells Chromatin Accessibility Data
ANALYSIS DESCRIPTION: Illumina DNAse-Seq read mappings from CD8 Primary Cells, Donor RO 01689, Library DS17332 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7779
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: Chromatin Accessibility
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 0bp
GENOMIC_WINDOW: 150bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 4
BROWSER_TRACK_NAME: CD8 DNase 27 32
BROWSER_TRACK_DESCRIPTION: UW CD8 Primary Cells DNase Hypersensitivity Donor RO 01689 Library DS17332 EA Release 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 25,588,179
NUMBER_OF_CHROMATIN_ACCESSIBILITY_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 52
FINDPEAKS_SCORE: 0.2729
FINDPEAKS_PERCENTILE: 13
HOTSPOT_SCORE: 0.2739
HOTSPOT_PERCENTILE: 11
IROC_SCORE: 0.9955
IROC_PERCENTILE: 63
POISSON_SCORE: 0.3195
POISSON_PERCENTILE: 9
MAXIMUM_REPLICATE_CORRELATION: 0.93
**********************************************************************
|
| |
|
| Submission date |
Apr 04, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Northwest REMC |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Washington
|
| Street address |
-
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (1) |
| GSE18927 |
University of Washington Human Reference Epigenome Mapping Project |
|
| Relations |
| SRA |
SRX055163 |
| BioSample |
SAMN00254258 |
| Named Annotation |
GSM701499_UW.CD8_Primary_Cells.ChromatinAccessibility.RO_01689.DS17332.wig.gz |
