|
| Status |
Public on Apr 14, 2011 |
| Title |
Chromatin accessibility assay of Fetal Muscle, Arm; DS17432 |
| Sample type |
SRA |
| |
|
| Source name |
Fetal primary tissue, day 115; DS17432
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: fMuscle_Arm.H-23808d115
sample common name: Fetal Muscle, Arm
molecule: genomic DNA
disease: None
biomaterial_provider: University of Washington, Congenital Defects Lab. Ian Glass
biomaterial_type: Primary Tissue
tissue_type: Fetal Muscle, Arm
tissue_depot: UW
collection_method: fetal
donor_id: H-23808
donor_age: day 115
donor_health_status: NA
donor_sex: Male
donor_ethnicity: NA
experiment_type: Chromatin Accessibility
extraction_protocol: Qiagen minElut
dnase_protocol: Stamlab DNase Protocol, Sabo, P. J. et al. Nat Methods 3, 511-518 (2006)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
DNase-Hypersensitivity |
| Library source |
genomic |
| Library selection |
DNAse |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
sample_term_id: UBERON_0001499
assay_term_id: OBI_0001853
nucleic_acid_term_id: SO_0000352
Design description: Chromatin accessibility determination via DNase-seq
Library name: DS17432
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FEXPERIMENT%2FEDACC.7272
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.7271
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM701506_UW.Fetal_Muscle_Arm.ChromatinAccessibility.H-23808.DS17432.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DS17432.hg19.level.1.release.4
ANALYSIS TITLE: Mapping of Fetal Muscle Arm Chromatin Accessibility Data
ANALYSIS DESCRIPTION: Illumina reads produced by DNAse-Seq on Fetal Muscle Arm, Donor H-23808, Library DS17432 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7714
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: Chromatin Accessibility
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: None
ALIGNMENT_POSTPROCESSING: None
RELEASE_NUMBER: Human Epigenome Atlas 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 30,970,938
NUMBER_OF_CHROMATIN_ACCESSIBILITY_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 54
FINDPEAKS_SCORE: 0.3798
FINDPEAKS_PERCENTILE: 46
HOTSPOT_SCORE: 0.3658
HOTSPOT_PERCENTILE: 44
IROC_SCORE: 0.992
IROC_PERCENTILE: 30
POISSON_SCORE: 0.4468
POISSON_PERCENTILE: 54
MAXIMUM_REPLICATE_CORRELATION: 0.91
**********************************************************************
ANALYSIS FILE NAME: GSM701506_UW.Fetal_Muscle_Arm.ChromatinAccessibility.H-23808.DS17432.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DS17432.hg19.level.2.release.4
ANALYSIS TITLE: Raw Signal Density Graphs of Fetal Muscle Arm Chromatin Accessibility Data
ANALYSIS DESCRIPTION: Illumina DNAse-Seq read mappings from Fetal Muscle Arm, Donor H-23808, Library DS17432 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7768
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: Chromatin Accessibility
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 0bp
GENOMIC_WINDOW: 150bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 4
BROWSER_TRACK_NAME: FMA DNase 08 32
BROWSER_TRACK_DESCRIPTION: UW Fetal Muscle Arm DNase Hypersensitivity Donor H-23808 Library DS17432 EA Release 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 30,970,938
NUMBER_OF_CHROMATIN_ACCESSIBILITY_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 52
FINDPEAKS_SCORE: 0.3798
FINDPEAKS_PERCENTILE: 46
HOTSPOT_SCORE: 0.3658
HOTSPOT_PERCENTILE: 44
IROC_SCORE: 0.992
IROC_PERCENTILE: 30
POISSON_SCORE: 0.4468
POISSON_PERCENTILE: 54
MAXIMUM_REPLICATE_CORRELATION: 0.91
**********************************************************************
|
| |
|
| Submission date |
Apr 04, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Northwest REMC |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Washington
|
| Street address |
-
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (2) |
| GSE18927 |
University of Washington Human Reference Epigenome Mapping Project |
| GSE51336 |
Mouse regulatory DNA landscapes reveal global principles of cis-regulatory evolution |
|
| Relations |
| SRA |
SRX055170 |
| BioSample |
SAMN00254285 |
| Named Annotation |
GSM701506_UW.Fetal_Muscle_Arm.ChromatinAccessibility.H-23808.DS17432.wig.gz |
