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| Status |
Public on Apr 14, 2011 |
| Title |
Chromatin accessibility assay of Fetal Muscle, Upper Limb Skeletal; DS17661 |
| Sample type |
SRA |
| |
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| Source name |
Fetal primary tissue, day 108; DS17661
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: fMuscle_Upper_limb_skeletal.H-23887d108
sample common name: Fetal Muscle, Upper Limb Skeletal
molecule: genomic DNA
disease: None
biomaterial_provider: University of Washington, Congenital Defects Lab. Ian Glass
biomaterial_type: Primary Tissue
tissue_type: Fetal Muscle, Upper Limb Skeletal
tissue_depot: UW
collection_method: fetal
donor_id: H-23887
donor_age: day 108
donor_health_status: NA
donor_sex: Female
donor_ethnicity: NA
experiment_type: Chromatin Accessibility
extraction_protocol: Qiagen minElut
dnase_protocol: Stamlab DNase Protocol, Sabo, P. J. et al. Nat Methods 3, 511-518 (2006)
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
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| Library strategy |
DNase-Hypersensitivity |
| Library source |
genomic |
| Library selection |
DNAse |
| Instrument model |
Illumina Genome Analyzer IIx |
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| Description |
sample_term_id: UBERON_0003662
assay_term_id: OBI_0001853
nucleic_acid_term_id: SO_0000352
Design description: Chromatin accessibility determination via DNase-seq
Library name: DS17661
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FEXPERIMENT%2FEDACC.7256
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.7255
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
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| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM701522_UW.Fetal_Muscle_Upper_Limb_Skeletal.ChromatinAccessibility.H-23887.DS17661.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DS17661.hg19.level.1.release.4
ANALYSIS TITLE: Mapping of Fetal Muscle Upper Limb Skeletal Chromatin Accessibility Data
ANALYSIS DESCRIPTION: Illumina reads produced by DNAse-Seq on Fetal Muscle Upper Limb Skeletal, Donor H-23887, Library DS17661 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7704
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: Chromatin Accessibility
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: None
ALIGNMENT_POSTPROCESSING: None
RELEASE_NUMBER: Human Epigenome Atlas 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 23,065,190
NUMBER_OF_CHROMATIN_ACCESSIBILITY_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 54
FINDPEAKS_SCORE: 0.4767
FINDPEAKS_PERCENTILE: 80
HOTSPOT_SCORE: 0.4759
HOTSPOT_PERCENTILE: 76
IROC_SCORE: 0.9945
IROC_PERCENTILE: 54
POISSON_SCORE: 0.5307
POISSON_PERCENTILE: 83
MAXIMUM_REPLICATE_CORRELATION: NA
**********************************************************************
ANALYSIS FILE NAME: GSM701522_UW.Fetal_Muscle_Upper_Limb_Skeletal.ChromatinAccessibility.H-23887.DS17661.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DS17661.hg19.level.2.release.4
ANALYSIS TITLE: Raw Signal Density Graphs of Fetal Muscle Upper Limb Skeletal Chromatin Accessibility Data
ANALYSIS DESCRIPTION: Illumina DNAse-Seq read mappings from Fetal Muscle Upper Limb Skeletal, Donor H-23887, Library DS17661 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7758
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: Chromatin Accessibility
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 0bp
GENOMIC_WINDOW: 150bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 4
BROWSER_TRACK_NAME: FMULS DNase 87 61
BROWSER_TRACK_DESCRIPTION: UW Fetal Muscle Upper Limb Skeletal DNase Hypersensitivity Donor H-23887 Library DS17661 EA Release 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 23,065,190
NUMBER_OF_CHROMATIN_ACCESSIBILITY_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 52
FINDPEAKS_SCORE: 0.4767
FINDPEAKS_PERCENTILE: 80
HOTSPOT_SCORE: 0.4759
HOTSPOT_PERCENTILE: 76
IROC_SCORE: 0.9945
IROC_PERCENTILE: 54
POISSON_SCORE: 0.5307
POISSON_PERCENTILE: 83
MAXIMUM_REPLICATE_CORRELATION: NA
**********************************************************************
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| Submission date |
Apr 04, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Northwest REMC |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Washington
|
| Street address |
-
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platform ID |
GPL10999 |
| Series (1) |
| GSE18927 |
University of Washington Human Reference Epigenome Mapping Project |
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| Relations |
| SRA |
SRX055186 |
| BioSample |
SAMN00254290 |
| Named Annotation |
GSM701522_UW.Fetal_Muscle_Upper_Limb_Skeletal.ChromatinAccessibility.H-23887.DS17661.wig.gz |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM701522_UW.Fetal_Muscle_Upper_Limb_Skeletal.ChromatinAccessibility.H-23887.DS17661.bed.gz |
324.7 Mb |
(ftp)(http) |
BED |
| GSM701522_UW.Fetal_Muscle_Upper_Limb_Skeletal.ChromatinAccessibility.H-23887.DS17661.wig.gz |
157.9 Mb |
(ftp)(http) |
WIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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