|
| Status |
Public on Sep 10, 2011 |
| Title |
PS2-2 iPSC |
| Sample type |
RNA |
| |
|
| Source name |
undifferentiated PS2-2 iPSC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC from primary fibroblast with presenilin 2 mutation, N141
|
| Treatment protocol |
Cells were washed with PBS and Rneasy kit was used to isolate total RNA.
|
| Growth protocol |
iPSC were cultured in hES medium containing bFGF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were isolated using RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Approximately 150 ng of RNA from each sample was labeled using GeneChip 3’IVT Express (Affymetrix) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, all arrays were hybridized at 45C for 16 hr.
|
| Scan protocol |
GeneChips were scanned using an AFX GC3000 G7 scanner.
|
| Data processing |
The gene expression raw data were extracted using the AFFX Gene Chip Operation System. Quality control was performed on the basis of Affymetrix quality control metrics. The data were analyzed with the Gene Spring GX 11.0 (Agilent).
|
| |
|
| Submission date |
Apr 04, 2011 |
| Last update date |
Jun 14, 2012 |
| Contact name |
Takuya Yagi |
| E-mail(s) |
[email protected]
|
| Organization name |
Keio University
|
| Street address |
35-Shinanomachi Shinjuku-ku
|
| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28379 |
Gene expression profiles of familial Alzheimer's disease with presenilin 2 mutation patient-specific induced pluripotent stem cells |
|
