|
| Status |
Public on Feb 27, 2023 |
| Title |
BAT, SSU72 aKO, Room temperature, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Brown adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Brown adipose tissue
Sex: male
genotype: ssu72 aKO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from adipose tissues using QIAzol Lysis Reagent as manufacturer's instruction. 5ug of total RNA was used for the construction of sequencing libraries.
RNA-Seq libraries were prepared with a TruSeq Stranded mRNA Library Prep kit (Illumina) using purified RNAs isolated from BAT of Ssu72 WT and aKO mice
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Transcriptomic sequencing was performed on a Novaseq platform. An initial sequence-level quality assessment was performed using FastQC. RNA-Seq reads were then mapped to the human mm10 reference genome using Tophat (v2.0.13). Assembly, quantification and differential expression analysis were performed using Cuffdiff (v2.2.1)
Assembly: mm10
Supplementary files format and content: tab-delimited text files include FPKM values
|
| |
|
| Submission date |
Feb 02, 2023 |
| Last update date |
Feb 28, 2023 |
| Contact name |
Eun-Ji Park |
| Organization name |
Sungkyunkwan University
|
| Street address |
2066 Seobu-ro
|
| City |
Suwon |
| ZIP/Postal code |
16419 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE224337 |
Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation |
|
| Relations |
| BioSample |
SAMN33018186 |
| SRA |
SRX19259291 |
