|
| Status |
Public on Apr 22, 2011 |
| Title |
CD4 14 CFA 0 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
CD4+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
treatment: CFA
strain: C57BL/6
|
| Treatment protocol |
Cells were either unstimulated (0) or stimulated in vitro for 14 days with 50 ug/ml of MOG peptide 35-55 (14)
|
| Growth protocol |
CD4+ T cells have been magnetics beads purified from peripheral lymph nodes of Cd57Bl/6 mice that has been immunized with 150 ug of MOG peptide 35-55 in CFA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mirVana kit (Ambion)
|
| Label |
Hy3
|
| Label protocol |
Our miRCURY™ LNA Array Power labeling kit allows highly efficient and uniform labeling of microRNAs with minimal sequence bias (Exiqon).
|
| |
|
| Channel 2 |
| Source name |
Common reference pool
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
|
| Treatment protocol |
Cells were either unstimulated (0) or stimulated in vitro for 14 days with 50 ug/ml of MOG peptide 35-55 (14)
|
| Growth protocol |
CD4+ T cells have been magnetics beads purified from peripheral lymph nodes of Cd57Bl/6 mice that has been immunized with 150 ug of MOG peptide 35-55 in CFA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mirVana kit (Ambion)
|
| Label |
Hy5
|
| Label protocol |
Our miRCURY™ LNA Array Power labeling kit allows highly efficient and uniform labeling of microRNAs with minimal sequence bias (Exiqon).
|
| |
|
| |
| Hybridization protocol |
Using Tecan hybridization stations the hybridization and washing steps are fully automated for excellent reproducibility (Exiqon).
|
| Scan protocol |
Arrays are scanned with highly sensitive equipment in ozone free environment to reduce day-to-day variation to a minimum. Image Analysis is then performed to quantify the signals on the arrays (Exiqon).
|
| Description |
Exiqon 10.0
|
| Data processing |
Normalization method: LOWESS. The data obtained is normalized using methods applicable to the performed experiment (Exiqon).
Depending on the experimental setup and the size of the project customized data analysis is provided (including heat map/Unsupervised Hierarchical Clustering, PCA-plots etc based on TTests or ANOVA) (Exiqon).
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Apr 22, 2011 |
| Contact name |
Marcin P Mycko |
| E-mail(s) |
[email protected]
|
| Phone |
4842 67577603
|
| Organization name |
Medical University of Lodz
|
| Department |
Department of Neurology
|
| Lab |
Laboratory of Neuroimmunology
|
| Street address |
Kopcinskiego 22
|
| City |
Lodz |
| ZIP/Postal code |
90-153 |
| Country |
Poland |
| |
|
| Platform ID |
GPL13387 |
| Series (1) |
| GSE28473 |
Quantitative analysis of miRNA changes in CD4+ T cells responding to myelin antigen |
|
