|
| Status |
Public on Apr 09, 2011 |
| Title |
Pool of 62 normal adrenal glands |
| Sample type |
RNA |
| |
|
| Source name |
AD_CTL
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal adrenal glands
tumor secretion profile: None
genotype: WT CTNNB1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 GeneCHIP.
|
| Scan protocol |
GeneChips were scanned using the Genechip scanner 3000 workstation (Affymetrix)
|
| Description |
Pool of total RNA from adrenal glands of 62 caucasian subjects aged 15 to 61 years old
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Apr 09, 2011 |
| Contact name |
julien durand |
| E-mail(s) |
[email protected]
|
| Phone |
514-890-8000
|
| Organization name |
CRCHUM
|
| Department |
Biomedicale Science
|
| Street address |
3840 Rue Saint-Urbain
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H2W 1T8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28476 |
Characterization of differential gene expression in adrenocortical tumors harbouring β-catenin (CTNNB1) mutations. |
|
