|
| Status |
Public on Mar 22, 2023 |
| Title |
Human cancer cells : Mouse stellate cells [Sample_4_M2] |
| Sample type |
SRA |
| |
|
| Source name |
Pancreas
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
tissue: Pancreas
cell line: Miapaca2, mPSC
cell type: Cancer Cell, Stellate Cell
genotype: WT
treatment: None
|
| Growth protocol |
Cancer and Stellate cells were formed into spheres, embedded in collagen : matrigel hydrogels and cultured for 72 hours in DMEM:F12 supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Monarch Total RNA Miniprep Kit (NEB), following manufacturers instructions.
RNA libraries for RNAseq was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Partek Flow Genomic Analysis Software
Data aligned to genome (Homo Sapiens Hg38, Mus Musculus mm10) - Star version STAR 2.7.3A
Read count extraction and normalization were performed using Partek software
Assembly: Hg38 , mm10
Supplementary files format and content: Excel file_Raw counts for each species
|
| |
|
| Submission date |
Feb 13, 2023 |
| Last update date |
Mar 22, 2023 |
| Contact name |
Ed Carter |
| E-mail(s) |
[email protected]
|
| Organization name |
Barts Cancer Institute
|
| Street address |
John Vane Science Centre
|
| City |
London |
| ZIP/Postal code |
EC1M6BQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19415 |
| Series (1) |
| GSE225161 |
Pancreatic stellate and cancer cell transcriptomes from heterocellular, 3D invasive, cultures |
|
| Relations |
| BioSample |
SAMN33269687 |
| SRA |
SRX19350840 |
