|
| Status |
Public on Jun 15, 2012 |
| Title |
Liver sample from Alcoholic hepatitis group (CA5) |
| Sample type |
RNA |
| |
|
| Source name |
liver biopsy sample
|
| Organism |
Homo sapiens |
| Characteristics |
liver sample group: : Alcoholic hepatitis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For total RNA extraction cryopreserved liver tissue samples were homogenized in TRIzol reagent (Invitrogen, San Diego, CA, USA) using pestle and nuclease-free 1,5ml reaction tubes (Ambion). Total RNA was then extracted following the manufacturers guidelines and quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
| Description |
Liver sample from Alcoholic hepatitis group
|
| Data processing |
Data processing cel files were normalized with 'gcrma' function of the Bioconductor package (v2.4) 'gcrma' in R.2.9.1
|
| |
|
| Submission date |
Apr 14, 2011 |
| Last update date |
Jun 15, 2012 |
| Contact name |
Juanjo Lozano |
| E-mail(s) |
[email protected]
|
| Organization name |
CIBEREHD
|
| Department |
Plataforma de Bioinformatica
|
| Street address |
C/ Rosselló 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28619 |
Transcriptome Analysis Identifies Fn14, a TNF Superfamily Receptor Member, as a Therapeutic Target in Alcoholic Hepatitis |
|
