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Sample GSM709569 Query DataSets for GSM709569
Status Public on Mar 06, 2012
Title preadipocytes, no differentiation, donor 1
Sample type RNA
 
Source name preadipocytes, no differentiation, donor 1
Organism Homo sapiens
Characteristics cell type: preadipocytes
time: 0 days of differentiation
individual: donor 1
Treatment protocol Differentiation into adipocytes was initiated by addition of 10 µg/ml insulin, 1 µM dexamethasone, 200 µM indomethacin and 500 µM isobutylmethylxanthine (Cambrex) to the culture medium.
Growth protocol Subcutaneous human preadipocytes were incubated in basal growth medium (Cambrex) containing 10% fetal calf serum, 2 mM L-glutamine, 100 U/ml penicilline and 100 µg/ml streptomycine (= culture medium).
Extracted molecule total RNA
Extraction protocol RNA was extracted using Trizol (Invitrogen) following manufacturer's instructions.
Label Cy3
Label protocol 1 µg of each RNA was used as template to produce Cy3- labeled cRNA. The RNA samples were amplified and labeled using the Agilent Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer’s protocol.
 
Hybridization protocol Cy3 labeled cRNAs were hybridized overnight (17 hours, 65°C) to an Agilent Whole Mouse Genome Oligo Microarrays (44K) using Agilent’s recommended hybridization chamber and oven.Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
Description female, 33 y, BMI 21.56, subcutaneous thighs
Data processing The Agilent Feature Extraction Software (FES 10.5.1.1 ) was used to read out and process the microarray image files.
 
Submission date Apr 14, 2011
Last update date Mar 06, 2012
Contact name Silvia Rueberg
Organization name Miltenyi Biotec GmbH
Department Genomic Services Department
Street address Friedrich-Ebert-Str. 68
City Bergisch Gladbach
ZIP/Postal code 51429
Country Germany
 
Platform ID GPL4133
Series (1)
GSE28628 Identification of new genes involved in human adipogenesis and fat storage

Data table header descriptions
ID_REF
VALUE median normalized signal intensities

Data table
ID_REF VALUE
12 3.96787
13 0.873461
14 6.523194
15 0.228973
16 45.00998
17 0.015392
18 2.078006
19 367.772521
20 0.198147
21 0.031363
22 257.351107
23 0.115404
24 9.37867
25 88.51331
26 0.165476
27 5.574636
28 0.015744
29 0.015788
30 58.769027
31 0.015884

Total number of rows: 43376

Table truncated, full table size 632 Kbytes.




Supplementary file Size Download File type/resource
GSM709569_251485059838_S01_GE1_105_Jan09_1_1.txt.gz 9.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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