|
| Status |
Public on Aug 20, 2005 |
| Title |
CBMNC.LPS+.3 |
| Sample type |
RNA |
| |
|
| Source name |
neonatal cord blood mononunclear cells after LPS-treatment
|
| Organism |
Homo sapiens |
| Characteristics |
Peripheral blood was obtained by venipuncture from healthy adult volunteers. Blood samples from umbilical cords of the placentas of healthy, full-term infants after scheduled caesarean section or vaginal delivery were obtained by puncture of the cord immediately after delivery
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 1.5 x 107 CBMNC or APBMNC with RNeasy Kit (Qiagen). Quantity and purity of extracted RNA was checked by RNA LabChip® Kit (Agilent).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated labeling cRNA was conducted by in vitro transcription using the BioArray High Yield RNA Transcript Labeling System (ENZO). cRNA was purified by GeneChip Sample Cleanup Module (Affymetrix).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was heated for 5 min at 95°C, allowing RNA to breakdown to small fragments according to Affymetrix protocols. Fragmented cRNA (20 µg) was subjected to oligonucleotide hybridization (Hybridization Oven; Affymetrix) to the human Genome Focus Genechip (Affymetrix).
|
| Description |
to evaluate in vitro activation of the neonatal and adult immune system, gene expression patterns were compared in mononuclear cells from cord and adult peripheral blood without and after incubation with 5 µg/ml Lipopolysaccharide (LPS).
|
| Data processing |
software package R, version 1.9.1, libraries gcrma and limma of the Bioconductor Project
|
| |
|
| Submission date |
Aug 19, 2005 |
| Last update date |
Oct 28, 2005 |
| Contact name |
Lutz Koch |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Heidelberg
|
| Department |
Department of Paediatrics
|
| Lab |
Division of Neonatology
|
| Street address |
Im Neuenheimer Feld 153
|
| City |
Heidelberg |
| ZIP/Postal code |
69115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL201 |
| Series (1) |
| GSE3140 |
Gene expression profiles of adult peripheral and cord blood mononuclear cells altered by lipopolysaccharide |
|
