|
| Status |
Public on Apr 15, 2023 |
| Title |
JAPC11_(Celegans)_hif-1 hypoxia_BioRep2 |
| Sample type |
RNA |
| |
|
| Source name |
hif-1(ia04) loss-of-function mutants
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
treatment: Hypoxia
|
| Treatment protocol |
The hypoxia treatment was 0.5% oxygen for 2 hours.
|
| Growth protocol |
Worms were grown on NGM plates seeded with OP50 at 21°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA isolation was performed using Trizol (Invitrogen) and RNeasy Mini Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
The total RNA was synthesized to biotin-labeled aRNA using the GeneChip® 3' IVT Express Kit (Affymetrix, part number 901229).
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix GeneChip® C. elegans Genome array according to Affymetrix specifications.
|
| Scan protocol |
The arrays were scanned with GeneChip® scanner 3000 7G.
|
| Description |
Gene expression
|
| Data processing |
The Affymetrix® GeneChip® Command Console™ (AGCC) software was used to generate probe cell intensity data (.CEL) files.
|
| |
|
| Submission date |
Apr 03, 2023 |
| Last update date |
Apr 16, 2023 |
| Contact name |
Jo Anne Powell-Coffman |
| E-mail(s) |
[email protected]
|
| Phone |
515-294-3906
|
| Organization name |
Iowa State University
|
| Department |
Department of Genetics, Development and Cell Biology
|
| Street address |
2213 Pammel Dr.
|
| City |
Ames |
| State/province |
Iowa |
| ZIP/Postal code |
50011-1101 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE228851 |
Gene expression under short-term moderate hypoxia and in the HIF-1 negative regulator mutants in Caenorhabditis elegans |
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