|
| Status |
Public on Jan 09, 2012 |
| Title |
PBMC from pNonTOL group (W89_pNonTOL) |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
tolerance group: pNonTOL
age: 67
gender [0=male;1=female]: 0
months from transplant: 57.83
cell type: PBMC
|
| Treatment protocol |
Ficoll PBMC isolation from peripheral blood
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from peripheral blood using the TRIzol method (Invitrogen, Cergy Pontoise, France). Genomic DNA was removed by DNase treatment (Roche, Indianapolis, IN, USA). RNA quality and quantity was determined using an Agilent 2100 BioAnalyzer (Palo Alto, CA, USA). RNA was reverse transcribed into cDNA using polydT oligonucleotide and Maloney leukemia virus reverse transcription (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
| Description |
PBMC from pNonTOL group
|
| Data processing |
Data processing cel files were normalized with 'frma' function of the Bioconductor package (v2.6) 'frma' in R.2.11.1
|
| |
|
| Submission date |
Apr 25, 2011 |
| Last update date |
Jan 09, 2012 |
| Contact name |
Juanjo Lozano |
| E-mail(s) |
[email protected]
|
| Organization name |
CIBEREHD
|
| Department |
Plataforma de Bioinformatica
|
| Street address |
C/ Rosselló 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28842 |
Withdrawal of immunosuppressive therapy in stable liver transplant recipients |
|
