Data table header descriptions |
ID_REF |
|
grid.r |
grid row |
grid.c |
grid column |
spot.r |
spot row in every grid |
spot.c |
spot column in every grid |
area |
number of pixels |
Gmean |
pixels mean intensity (green) |
Gmedian |
pixels median intensity (green) |
GIQR |
inter quartile range (green) (dispersion of 50% of pixels closer to median) |
Rmean |
pixels mean intensity (red) |
Rmedian |
pixels median intensity (red) |
RIQR |
inter quartile range (red) (dispersion of 50% of pixels closer to median) |
bgGmean |
mean background intensity (green) |
bgGmed |
median background intensity (green) |
bgGSD |
background standard deviation (green) |
bgRmean |
mean background intensity (red) |
bgRmed |
median background intensity (red) |
bgRSD |
background standard deviation (red) |
valleyG |
mean of the background median for 4 diamond areas around the spot (green) (spot program for details) |
valleyR |
mean of the background median for 4 diamond areas around the spot (red) (spot program for details) |
morphG |
background estimation using morphological opening (green) (spot program for details) |
morphG.erode |
background estimation using morphological erosion (green) (spot program for details) |
morphG.close.open |
background estimation using morphological closing-opening (green) (spot program for details) |
morphR |
background estimation using morphological opening (red) (spot program for details) |
morphR.erode |
background estimation using morphological erosion (red) (spot program for details) |
morphR.close.open |
background estimation using morphological closing-opening (red) (spot program for details) |
logratio |
log2((Rmedian-morphR)/(Gmedian-morphG)) |
perimeter |
2*sqrt(pi*area/circularity) |
circularity |
4*pi*area/perimeter^2 |
badspot |
if 0 -> good spot |
nominal.center.row |
nominal center row |
nominal.center.col |
nominal center column |
VALUE |
final filtered and normalized log2ratio |