|
| Status |
Public on Apr 27, 2012 |
| Title |
VCAP, 24h 10nM DHT |
| Sample type |
RNA |
| |
|
| Source name |
VCaP prostate cancer cells, 24h DHT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: VCaP
cell type: prostate cancer cells
treatment: 10nM dihydrotestosterone (DHT)
treatment duration: 24h
|
| Treatment protocol |
VCaP cells were seeded in DMEM supplemented with 10% fetal bovine serum (FBS), sodium pyruvate, sodium bicarbonate and penicillin:streptomycin solution for 3 days. Prior to ETOH/DHT stimulation, VCaP cells were washed and the medium was replaced with phenol red free DMEM supplemented with 10% charcoal stripped fetal bovine serum (CDFBS), sodium pyruvate, sodium bicarbonate and penicillin:streptomycin solution to starve the cells for 24 hours. Hormone-deprived VCaP cells were treated with ETOH/10nM DHT for different times.
|
| Growth protocol |
The human prostate cancer VCaP cells (American Type Culture Collection) were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), sodium pyruvate, sodium bicarbonate and penicillin:streptomycin solution at 37˚C under 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Sigma) and purified according to the manufacturer's protocol in the PureLink RNA Mini Kit (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 500ng total RNA using the Illumina TotalPrep-96 RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
750ng of cRNA were hybridized to the Sentrix HumanRef-8 v3 Expression BeadChip Kit (Illumina) according to the manufacturer's protocol.
|
| Scan protocol |
BeadChips were scanned using the BeadArray Reader.
|
| Description |
This Sample represents triplicates.
Raw data IDs: 4671881007_A, 4671881104_A, 4671881105_A
|
| Data processing |
Raw data was analyzed using GeneSpring GX 11.0 software. The dataset was normalized using percentile shift normalization and baseline-transformed to the median of control samples. Analyses were performed using the average of the triplicates (after normalization).
|
| |
|
| Submission date |
Apr 28, 2011 |
| Last update date |
Apr 27, 2012 |
| Contact name |
Kern Rei Chng |
| E-mail(s) |
[email protected]
|
| Organization name |
GIS
|
| Department |
Cancer Biology and Pharmacology 3
|
| Lab |
Cancer Biology and Pharmacology 3
|
| Street address |
60 Biopolis Street #02-01 Genome
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6883 |
| Series (2) |
| GSE28948 |
TMPRSS2-ERG, HDACs and EZH2 are involved in an AR-centric transcriptional circuitry that calibrates androgenic response for prostate cancer progression (gene expression data) |
| GSE28951 |
TMPRSS2-ERG, HDACs and EZH2 are involved in an AR-centric transcriptional circuitry that calibrates androgenic response for prostate cancer progression |
|
