|
| Status |
Public on Aug 29, 2005 |
| Title |
CF634 |
| Sample type |
RNA |
| |
|
| Source name |
cultured fibroblast - individual 634
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue type: epithelium
Sample type: cultured fibroblast
idv ID: 634
Donor Age: 23
Site: breast
indivRep: 634
|
| Extracted molecule |
total RNA |
| Label |
biotin
|
| Label protocol |
standard Affymetrix
|
| |
|
| Description |
epithelium cell type comparison
|
| Data processing |
We used a normalization and referencing strategy that consisted of BioConductor/RMA Express RMA processing of the entire series of cel files followed by a per gene normalization in which the median value of expression for each gene was derived from the cultured samples only, and this was used as a reference for all samples including the cultured skin substitute. This approach allowed for the identification of genes that were higher and lower-expressed in the cultured skin relative to the individual cell types that were also expressed strongly or weakly in normal skin relative to the median value established by the three cell types.
|
| |
|
| Submission date |
Aug 25, 2005 |
| Last update date |
Sep 15, 2005 |
| Contact name |
Bruce J Aronow |
| E-mail(s) |
[email protected]
|
| Phone |
513-636-4865
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE3204 |
Profile of cultured skin substitute relative to constituent keratinocytes and fibroblasts and in relation to normal skin |
|
