|
| Status |
Public on May 16, 2011 |
| Title |
TMK-1 cells stably expressing miR-NRDc clone #1 |
| Sample type |
RNA |
| |
|
| Source name |
TMK-1 gastric cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: gastric adenocarcinoma
cell line: TMK-1 cells stably expressing miR-NRDc
|
| Treatment protocol |
TMK-1 clones stablly expressing control vector (miR-NC, non-targeting sequence) or miR against NRDc (target sequence: 5’-CTGATGCAAACAGAAAGGAAA-3’) were established by selection with blastcidin.
|
| Growth protocol |
TMK-1 gastric cancer cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum and 5 mg/ml blastcidin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy mini kit (QIAGEN) and digested with DNase.
|
| Label |
biotin
|
| Label protocol |
Biotinylated sense-strand DNA was generated from 100 ng of total RNA with WT Sense Target Labeling and Control Reagent (Affymetrix).
|
| |
|
| Hybridization protocol |
Sense-strand DNA was hybridized on Affymetrix GeneChip Human Gene 1.0 ST Array.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 7G System.
|
| Description |
Reduced expressin of NRDc by RNAi
|
| Data processing |
Microarray data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and GeneSpring GX Version 10.0.2 (Agilent Technologies) and normalized by RMA16 algorithm.
|
| |
|
| Submission date |
May 09, 2011 |
| Last update date |
May 16, 2011 |
| Contact name |
Hideyuki Komekado |
| E-mail(s) |
[email protected]
|
| Phone |
+81-75-751-4319
|
| Fax |
+81-75-751-4303
|
| Organization name |
Graduate School of Medicine, Kyoto University
|
| Department |
Gastroenterology and Hepatology
|
| Street address |
54 Kawara-cho, Shogoin, Sakyo-ku,
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE29144 |
Gene expression profiles after knockdown of Nardilysin in TMK-1 gastric cancer cells |
|
