|
Status |
Public on Nov 10, 2005 |
Title |
germ cell tumors (GCT) and normal controls Norm1B2 1 |
Sample type |
RNA |
|
|
Source name |
normal testis control
|
Organism |
Homo sapiens |
Characteristics |
normal testis
|
Extracted molecule |
total RNA |
Extraction protocol |
Frozen tissue from normal testis was minced and homogenized in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Approximately 12 ug of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Normal Testis Specimen
|
Data processing |
RMA (robust multi-array)
|
|
|
Submission date |
Aug 29, 2005 |
Last update date |
Oct 12, 2005 |
Contact name |
James Korkola |
E-mail(s) |
[email protected]
|
Phone |
212-639-8281
|
Organization name |
Memorial Sloan-Kettering
|
Department |
Cell Biology
|
Lab |
Chaganti
|
Street address |
1275 York Ave
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL97 |
Series (1) |
GSE3218 |
Expression Profiling of Adult Male Germ Cell Tumors |
|