|
| Status |
Public on Dec 29, 2011 |
| Title |
floral bud sample from May 2006, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
male floral buds Populus deltoides sampled in 2011
|
| Organism |
Populus deltoides |
| Characteristics |
tissue: bud
age: sexually mature tree
collection date: May
block: 1
|
| Treatment protocol |
NA, samples represent wild-type tissues
|
| Growth protocol |
Samples were collected from a 30-year old male Populus deltoides tree growing next to a stream. Samples were taken for months April through August at the end of the second week of the month.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with a hot-borate method (Wan and Wilkins; 1994) followed by a Dnase I digestion and clean-up using Rneasy Mini Kit (Quigen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Poplar Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Genechip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression of preformed leaf tissue
|
| Data processing |
Pre-processing was done using the Robust Multichip Average using Affy package version 1.6 in the VioC 1.8 release of Bioconductor and run in the R environment.
|
| |
|
| Submission date |
May 17, 2011 |
| Last update date |
Dec 29, 2011 |
| Contact name |
Joshua Adams |
| E-mail(s) |
[email protected]
|
| Phone |
8704601348
|
| Organization name |
University of Arkansas-Monticello
|
| Department |
Forest Resources
|
| Street address |
PO Box 3468
|
| City |
Monticello |
| State/province |
AR |
| ZIP/Postal code |
71656 |
| Country |
USA |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE29335 |
Time-course bud expression data from Populus deltoides |
|
