|
| Status |
Public on Nov 10, 2005 |
| Title |
germ cell tumors (GCT) and normal controls NormPool 2 |
| Sample type |
RNA |
| |
|
| Source name |
pooled normal testis control
|
| Organism |
Homo sapiens |
| Characteristics |
normal testis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen tissue from normal testis was minced and homogenized in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 12 ug of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
pooled testis specimen (consists of equal amounts of norm1B1, norm1B2, norm1B3, norm1B4, and norm1C10)
|
| Data processing |
RMA (robust multi-array)
|
| |
|
| Submission date |
Aug 29, 2005 |
| Last update date |
Oct 12, 2005 |
| Contact name |
James Korkola |
| E-mail(s) |
[email protected]
|
| Phone |
212-639-8281
|
| Organization name |
Memorial Sloan-Kettering
|
| Department |
Cell Biology
|
| Lab |
Chaganti
|
| Street address |
1275 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE3218 |
Expression Profiling of Adult Male Germ Cell Tumors |
|
