|
| Status |
Public on Dec 08, 2011 |
| Title |
P2 fat tissue |
| Sample type |
RNA |
| |
|
| Source name |
developing adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: developing adipose tissue
developmental stage: P2
|
| Treatment protocol |
RNA isolated from developing WAT was pooled from four independent embryos at eight different embryonic and early postnatal time points. The RNA was then labeled and hybridized to Illumina Mouse Ref8 BeadChips.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from each sample was labeled using the Illumina TotalPrep RNA Amplification kit (Ambion) and cRNA was hybridized to Illumina's MouseRef-8 Expression BeadChips (Illumina). The arrays were washed, blocked, and then hybridized. Image processing and data extraction were performed using BeadStudio (Illumina).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Developing mouse adipose tissue pooled from at least 3 embryos
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
May 24, 2011 |
| Last update date |
Dec 08, 2011 |
| Contact name |
kivanc birsoy |
| E-mail(s) |
[email protected]
|
| Organization name |
Rockefeller University
|
| Street address |
1230 Rockefeller University
|
| City |
new york |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE29502 |
Gene expression profile of developing adipose tissue in vivo |
|
