|
| Status |
Public on May 25, 2011 |
| Title |
CD34+CD45RA+CD10+(CD7-CD19-) donor-2 |
| Sample type |
RNA |
| |
|
| Source name |
CD34+CD45RA+CD10+(CD7-CD19-) HPCs
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: umbilical cord blood
cell type: hematopoietic progenitors
|
| Treatment protocol |
CD34+ PCs were labelled with CD34, CD45RA, CD7, CD10 and CD19 antibodies before the corresponding population were sorted by FACS
|
| Growth protocol |
CD34+ HPCs were isolated from Umbilical cord blood midiMACS system (Miltenyi)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted using the RNeasy Micro Kit
|
| Label |
biotin
|
| Label protocol |
RNA samples were subjected to double linear amplification using the GeneChip Eukaryotic Small Sample Target Labeling Assay Version II (Affymetrix).
|
| |
|
| Hybridization protocol |
cRNAs were then hybridized to Affymetrix HG-U133A GeneChip arrays
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray Scanner 2500A
|
| Description |
Galy2
Gene expression data from pre-proB cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 25, 2011 |
| Last update date |
May 25, 2011 |
| Contact name |
Marc Delord |
| E-mail(s) |
[email protected]
|
| Organization name |
Centre hospitalier de Versailles
|
| Department |
Recherche clinique
|
| Street address |
Hopital Saint-Louis, 1 avenue Claude Vellefaux
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE29522 |
Expression data from human CD34+ HPC subpopulations isolated from umbilical cord blood (Haddad et al. Blood 104:3918, 2004) |
|
