|
| Status |
Public on May 01, 2012 |
| Title |
Proband3 18h in vitro |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T-Cells from peripheral blood of proband3 after 18h in vitro
|
| Organism |
Homo sapiens |
| Characteristics |
time: 18h
tissue: peripheral blood
cell type: CD4+ T-Cells
proband age: 25 years
proband sex: male
|
| Growth protocol |
After venipuncture at 10.30 o'clock the cells were cultivated at 12.00 o'clock.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated sense-strand DNA was generated from 100 ng of total RNA with WT Sense Target Labeling and Control Reagent (Affymetrix).
|
| |
|
| Hybridization protocol |
Sense-strand DNA was hybridized on Affymetrix GeneChip Human Gene 1.0 ST Array.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 System.
|
| Description |
CD4+ T-Cells from peripheral blood of proband3 after 18h in vitro
|
| Data processing |
Microarray data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix). The advanced analysis was done with R (version 2.9) with package limma.
|
| |
|
| Submission date |
May 27, 2011 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
[email protected]
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Pathology
|
| Lab |
NGS Integrative Genomics
|
| Street address |
Kreuzbergring 57
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37075 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE29583 |
Circadian Clock Activity in Mouse and Human CD4+ T Cells |
|
| Relations |
| Reanalyzed by |
GSE86357 |
