|
| Status |
Public on Jun 09, 2011 |
| Title |
Liver_BDL_1day_BDL12 |
| Sample type |
RNA |
| |
|
| Source name |
liver tissure, BDL 1 day
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
gender: male
age: adult
strain: C57BL/6J
treatment: cholestatic injury
|
| Treatment protocol |
Mouse Model of Extrahepatic Cholestasis: Eight- to 10-week-old (22-26 g) male C57BL/6J WT (#0664) and homozygous C57BL/6J PAI-1−/− mice (#2507) were obtained from the Jackson Laboratory (Bar Harbor, ME). The common bile duct was double ligated below the bifurcation, single ligated above the pancreas, and transected between the ligatures under methoxyflurane (Schering-Plough Co, Union, NJ) anesthesia. Sham operated mouse controls underwent a similar laparotomy without bile duct ligation.
|
| Growth protocol |
The use and care of mice were accredited and approved by the Washington University Animal Care Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated using TRI Reagent (Sigma, St. Louis, MO) was purified with an RNeasy mini kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Chips labeled with biotin according to Manufacturer's protocol
|
| |
|
| Hybridization protocol |
Chips hybridized according to Manufacturer's protocol
|
| Scan protocol |
GS3000
|
| Description |
Gene expression data from liver tissue at 1 day of cholestatic injury
|
| Data processing |
Data corrected to 1500 Baseline
|
| |
|
| Submission date |
Jun 07, 2011 |
| Last update date |
Jun 09, 2011 |
| Contact name |
Rekha Meyer |
| E-mail(s) |
[email protected]
|
| Phone |
314 362 8853
|
| Organization name |
Washington University School of Medicine
|
| Street address |
4320 Forest Park Av
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63128 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE29776 |
Transcriptional profiling after bile duct ligation identifies PAI-1 as a contributor to cholestatic injury in mice. |
|
