|
| Status |
Public on Jul 11, 2011 |
| Title |
3 uM EPZ004777 treated, DAY 2, biological rep.2, MV4-11 cells |
| Sample type |
RNA |
| |
|
| Source name |
MV4-11 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 2
biological rep: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
|
| Label |
biotin
|
| Label protocol |
RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
|
| Scan protocol |
Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
|
| Description |
Gene expression data from 3 uM EPZ004777 treated, DAY 2, biological rep.2, MV4-11 cells
|
| Data processing |
Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
|
| |
|
| Submission date |
Jun 08, 2011 |
| Last update date |
Jul 11, 2011 |
| Contact name |
Jesse Jerome Smith |
| E-mail(s) |
[email protected]
|
| Phone |
617-500-0596
|
| URL |
http://www.epizyme.com
|
| Organization name |
Epizyme
|
| Department |
Biological Sciences
|
| Street address |
325 Vassar Street, Suite 2B
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29828 |
Expression Profiling of Mixed Lineage Leukemia Cells Treated with a Potent Small-Molecule DOT1L Inhibitor |
|
