|
| Status |
Public on Jun 09, 2011 |
| Title |
Mixture (33:67), biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Mixed sample, percent.blood:percent.breast: 33%:67%
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: 33% blood and 67% breast
gender: female
developmental stage: adult
|
| Treatment protocol |
No treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol and RNeasy according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human GeneChip HG-U133_Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner GCS3000.
|
| Description |
Exploratory expression profiling.
|
| Data processing |
The data were analyzed with Robust Multiarray Average (RMA) normalization and scaled to a 2% trimmed mean of 150 using R/Bioconductor.
|
| |
|
| Submission date |
Jun 08, 2011 |
| Last update date |
Jun 09, 2011 |
| Contact name |
Ting Gong |
| Organization name |
Novartis Institutes for Biomedical research
|
| Street address |
45 Sidney street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29832 |
Expression data from pure/mixed blood and breast to test feasability of deconvolution of clinical samples |
|
