|
| Status |
Public on Aug 01, 2011 |
| Title |
Beta-iPS Cells clone 2 |
| Sample type |
RNA |
| |
|
| Source name |
iPS cells from pancreatic beta cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
cell type: undifferentiated induced pluripotent stem cells (iPSCs)
cell type derived from: pancreatic islet beta cells
|
| Treatment protocol |
Total RNA was isolated from undifferentiated pluripotent stem cells grown in standard HESC growth conditions on a mouse embryonic fibroblast feeder layer.
|
| Growth protocol |
Cells were maintained on mitomycin C (MMC)-inactivated mouse embryonic fibroblast (MEF) feeder layers in hESC medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the BiPSCs and PiPSCs was isolated using the Qiagen RNeasy mini kit.
|
| Label |
biotin
|
| Label protocol |
200ng total RNA, Affymetrix protocol.
|
| |
|
| Hybridization protocol |
11ug cRNA, Affymetrix protocol.
|
| Scan protocol |
GeneChip scanner 3000 7G, GeneChip Operating Software v1.4.
|
| Description |
BiPSCs #2
Gene expression data from Beta-iPS #2.
|
| Data processing |
Data were RMA normalized using Affymetrix Expression Console.
|
| |
|
| Submission date |
Jun 09, 2011 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Ori Bar-Nur |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard University
|
| Department |
Center of Regenerative medicine
|
| Lab |
Hochedlinger
|
| Street address |
185 Cambridge st.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE29880 |
Expression data from epigenetic memory and preferential lineage-specific differentiation in induced pluripotent stem cells derived from human pancreatic islet beta cells |
|
