|
| Status |
Public on Jun 21, 2011 |
| Title |
mid-L3_12 hour high sugar fed_biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
whole male Canton-S Drosophila mid-third instar larvae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole larva
genotype: Canton-S
developmental stage: mid-third instar
gender: male
agent: high sugar
|
| Treatment protocol |
Animals harvested as wandering L3 were fed control or high sucrose Semi-Defined food from embryo hatch. Animals harvested as mid-L3 were fed control food from embryo hatch, then transferred to fresh control or high sucrose as early L3 and aged 12 hours before harvest.
|
| Growth protocol |
Canton S were reared at 25C on Bloomington Semi-Defined Food with sucrose substituted for glucose (0.15M). High sugar food contained 1M sucrose instead of 0.15M.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tripure extraction of total RNA was performed according to the manufacturer's instructions; RNA was DNase-treated and then cleaned on a Qiagen RNeasy column and eluted in RNase-free H2O.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according tot the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
15 ug of biotinylated cRNAs were hybridized to Affymetrix Drosophila 2.0 oligonucleotide arrays.
|
| Scan protocol |
Gene chips were scanned using the GeneChip GS3000.
|
| Description |
14080
Gene expression data from actively feeding, mid-L3 larvae.
|
| Data processing |
The data were analyzed with dChip (2006 version) and precise match/mismatch probe intensity was used as normalization method.
|
| |
|
| Submission date |
Jun 16, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Thomas John Baranski |
| E-mail(s) |
[email protected]
|
| Organization name |
Washington University School of Medicine
|
| Street address |
660 S. Euclid Ave. Box 8127
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE30020 |
Expression data from control and high sugar-fed third instar Drosophila larvae |
|
| Relations |
| Reanalyzed by |
GSE119084 |
