|
| Status |
Public on Dec 05, 2023 |
| Title |
JIMT-1_HUNK KD1_1 |
| Sample type |
RNA |
| |
|
| Source name |
Human HER2+ Breast Cancer Cells (JIMT-1)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: JIMT-1
cell type: HER2+ Breast Cancer
|
| Treatment protocol |
HSL samples were treated with 1ug/ul of HSL119
|
| Growth protocol |
Cells grown in DMEM (Corning 10-017-CV) supplemented with 10% fetal bovine serum, 200 nM glutamine (Thermo Scientific), and penicillin/streptomycin (Thermo Scientific). HUNK knockdown cells were grown in media with 1ug/ul puromycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Used GeneJET RNA purifcation kit (K0732) to isolate RNA from cells.
|
| Label |
n/a
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Data processing |
NanoString nSolver 4.0 software was used for analysis of gene expression values
Nanostring nSolver 4.0 software computed normalized log2 count and normalized linear count
|
| |
|
| Submission date |
May 26, 2023 |
| Last update date |
Dec 05, 2023 |
| Contact name |
Elizabeth Yeh |
| E-mail(s) |
[email protected]
|
| Organization name |
Indiana University School of Medicine
|
| Department |
Pharmacology and Toxicology
|
| Street address |
635 Barnhill Drive, Medical Science Building A418
|
| City |
Indianapolis |
| State/province |
Indiana |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL33436 |
| Series (1) |
| GSE233608 |
HSL119 results in gene expression changes equivalent to HUNK shRNA |
|
