|
| Status |
Public on Oct 01, 2005 |
| Title |
Wildtype_24H Cold_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Columbia ecotype containing CBF3 promoter-driven luciferase transgene. As described in Chinnusamy, V., Ohta, M., Kanrar, S., Lee, B.H., Hong, X.H., Agarwal, M., and Zhu, J.K. (2003). ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes Dev. 17, 1043-1054.
|
| Treatment protocol |
Treated at 0C for 24 hours just prior to harvesting.
|
| Growth protocol |
The wild-type and ice1 mutant seeds were plated on MS agar plates supplemented with 3% sucrose. Seedlings were grown at 22C with 16-h light and 8-h dark cycles for 2 weeks before being treated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit, Qiagen
|
| Label |
Biotin
|
| Label protocol |
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
|
| |
|
| Hybridization protocol |
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
|
| Scan protocol |
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
|
| Description |
Target value (TGT value) was set to 500 global scaling.
|
| Data processing |
Microarray data were extracted from scanned GeneChip images and analyzed by use of Microarray Suite version 5.0.1
|
| |
|
| Submission date |
Sep 18, 2005 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jian-Kang Zhu |
| E-mail(s) |
[email protected]
|
| Phone |
951-827-7117
|
| Fax |
951-827-7115
|
| URL |
http://www.faculty.ucr.edu/~jkzhu
|
| Organization name |
University of California
|
| Department |
Botany and Plant Sciences
|
| Street address |
2150 Batchelor Hall
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE3326 |
ICE1 regulation of the Arabidopsis Cold-Responsive Transcriptome |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
