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Status
Public on Oct 01, 2005
Title
ice1_no treatment_Rep1
Sample type
RNA
Source name
Seedlings
Organism
Arabidopsis thaliana Characteristics
A mutant defective in an upstream transcription factor required for chilling and freezing tolerance as described in Chinnusamy, V., Ohta, M., Kanrar, S., Lee, B.H., Hong, X.H., Agarwal, M., and Zhu, J.K. (2003). ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes Dev. 17, 1043-1054.
Growth protocol
The wild-type and ice1 mutant seeds were plated on MS agar plates supplemented with 3% sucrose. Seedlings were grown at 22C with 16-h light and 8-h dark cycles for 2 weeks before being treated.
Extracted molecule
total RNA
Extraction protocol
RNeasy Plant Mini Kit, Qiagen
Label
Biotin
Label protocol
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
Hybridization protocol
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
Scan protocol
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
Description
Target value (TGT value) was set to 500 global scaling.
Data processing
Microarray data were extracted from scanned GeneChip images and analyzed by use of Microarray Suite version 5.0.1
Submission date
Sep 18, 2005
Last update date
Aug 28, 2018
Contact name
Jian-Kang Zhu
E-mail(s)
[email protected] Phone
951-827-7117
Fax
951-827-7115
URL
http://www.faculty.ucr.edu/~jkzhu Organization name
University of California
Department
Botany and Plant Sciences
Street address
2150 Batchelor Hall
City
Riverside
State/province
CA
ZIP/Postal code
92521
Country
USA
Platform ID
GPL198 Series (1)
GSE3326 ICE1 regulation of the Arabidopsis Cold-Responsive Transcriptome
Relations Reanalyzed by
GSE118579 Reanalyzed by
GSE119083
Data table header descriptions ID_REF VALUE ABS_CALL Presence or Absence
DETECTION P-VALUE
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
177.2
P
0.001796
AFFX-BioB-M_at
211
P
0.000044
AFFX-BioB-3_at
155
P
0.00006
AFFX-BioC-5_at
587.5
P
0.00007
AFFX-BioC-3_at
357.9
P
0.000044
AFFX-BioDn-5_at
742.8
P
0.000044
AFFX-BioDn-3_at
2835.5
P
0.000044
AFFX-CreX-5_at
6730.6
P
0.000044
AFFX-CreX-3_at
8419
P
0.000044
AFFX-DapX-5_at
16.3
A
0.300606
AFFX-DapX-M_at
22.1
A
0.327079
AFFX-DapX-3_at
2
A
0.987453
AFFX-LysX-5_at
10.7
A
0.262827
AFFX-LysX-M_at
27.6
A
0.559354
AFFX-LysX-3_at
25.9
P
0.004998
AFFX-PheX-5_at
5
A
0.749204
AFFX-PheX-M_at
1.1
A
0.904333
AFFX-PheX-3_at
7.3
A
0.699394
AFFX-ThrX-5_at
10.1
A
0.544587
AFFX-ThrX-M_at
10.6
A
0.51489
22810 589 Kbytes .Supplementary file Size Download File type/resource GSM74902.CEL.gz
3.4 Mb
(ftp) (http) CEL
GSM74902.EXP.gz
483 b
(ftp) (http) EXP
